論著(zhù)
纖維粘連蛋白和整合素β1在人腦膠質(zhì)瘤中的表達及其意義
趙杰 劉躍亭*
【摘要】 目的 研究纖維粘連蛋白、整合素β1在膠質(zhì)瘤中的表達及其與惡性程度的相關(guān)性，探討纖維粘連蛋白、整合素β1在人腦膠質(zhì)瘤的診斷及惡性程度判斷中的臨床意義。方法 應用免疫組織化學(xué)的方法檢測60例腦膠質(zhì)瘤組織標本、15例正常腦組織標本中纖維粘連蛋白、整合素β1的表達。
【關(guān)鍵字】 膠質(zhì)瘤,纖維粘連蛋白,整合素β1
中圖分類(lèi)號：R739.41文獻標識碼：B文章編號：1671-8194（2011）01-0025-03
The Expression and Significance of Fibronectin and Integrin β1 in Human Brain Glioma
ZHAO Jie, LIU Yue-ting
(Department of Neurosurgery, First Hospital of Shanxi Medical University, Taiyuan 030001, China)
[Abstract] Purpose To explore the expression of fibronectin and integrinβ1 in human glioma cell of the patientswith neurogliocytoma and to see whether or not fibronectin and integrinβ1 expression associate with thedifferentiation and pathologic grading. It was expected that this study could reveal some essentialqualities of the glioma and provide potential application for judging the malignant grades andbiological treatment. Methods The expression of fibronectin and integrinβ1 was detected in 60 cases of human glioma and 15 specimens of normal brain tissues by immunohistochemistry staining. Result Normal brain tissue specimens of Fibronectin and integrin β1 did not express.The positive rates of fibronectin were 31.67% (19/60) in glioma.The positive rates of fibronectin increased along with the elevation of glioma malignancy(P<0.05). The positive rates of integrinβ1 were 40.00%(24/60) in glioma.The positive rates of integrinβ1 increased along with the elevation of glioma malignancy(P<0.05). Significant positive correlation was observed between the expression of fibronectin and integrinβ1(r=0.518, P<0.05). Conclusion The positive correlation existed between the expression of fibronectin and integrinβ1 and the pathological grading of the human glioma.Detecting fibronectin and integrinβ1 in the human glioma can be used in diagnosing, judging the malignancy degree and the prognosis.
膠質(zhì)瘤是人類(lèi)中樞神經(jīng)系統最常見(jiàn)的腫瘤，它的主要生物學(xué)特性為局部侵襲性生長(cháng)而罕見(jiàn)顱外轉移。腦膠質(zhì)瘤細胞的侵襲性生長(cháng)和復發(fā)是膠質(zhì)瘤臨床治療失敗的主要原因。研究發(fā)現，黏附分子家族中的整合素參與了腫瘤細胞的黏附和遷移等多個(gè)環(huán)節，通過(guò)介導細胞與細胞外基質(zhì)蛋白、細胞之間的黏附和信號轉導等作用，直接和間接地影響腫瘤細胞的侵襲性，包括膠質(zhì)瘤細胞的侵襲性[1]。整合素家族的配體主要是細胞外基質(zhì)成分。在正常腦組織中，血管基底膜和膠質(zhì)界膜處的細胞外基質(zhì)主要包括W型膠原蛋白、纖維粘連蛋白(FN)、層粘連蛋白(LN)和玻粘連蛋白等[2，3]。我們采用免疫組織化學(xué)方法檢測了腦膠質(zhì)瘤中整合素β1、纖維粘連蛋白表達，了解二者在腦膠質(zhì)瘤組織中的表達情況，并分析它們與腫瘤分化程度及侵襲轉移的關(guān)系。
